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MAK038

Sigma-Aldrich

NADP/NADPH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):

NADP/NADPH Assay Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.25

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−70°C

General description

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.

Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.

Suitability

Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates

Principle

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H317,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell death & disease, 9(5), 572-572 (2018-05-16)
Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has a fundamental role in cancer growth and metastasis. Even though PPP blockade has been pointed out as a very promising strategy against cancer, effective anti-PPP agents are not
Q Shi et al.
Toxicology, 381, 64-74 (2017-02-28)
It has been well established that inflammation and concurrent mutagenic exposures drive the carcinogenic process in a synergistic way. To elucidate the role of the inflammatory cytokine IL-8 in this process, we studied its effect on the activation and deactivation
Mechanisms of action of isoniazid.
Timmins G S and Vojo D
Molecular Microbiology, 62(5), 1220-1227 (2006)
Andreas Carstensen et al.
Plant physiology, 177(1), 271-284 (2018-03-16)
Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that P deficiency affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how
Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase.
Berri'os-Rivera S J, et al.
Metabolic engineering, 4(3), 217-229 (2002)
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