The 1000 mL or 1L package size contains 42.4 grams of powdered medium which is sufficient to prepare 1 liter of liquid medium. Likewise, the 10L package size contains 424 grams of powdered medium which is sufficient to prepare 10 liters of liquid medium. Please see the link below to review the complete product formulation:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Media%20including%20Organics
M9274
Murashige and Skoog Basal Medium
suitable for plant cell culture, with sucrose and agar
Synonym(s):
MS Basal Medium
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| Size/SKU | Availability | Price |
|---|---|---|
1 L | Please contact Customer Service for Availability | 383,00 CZK |
10 L | Please contact Customer Service for Availability | 2 350,00 CZK |
About This Item
Quality Segment
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
Application
Preparation Note
Formulated to contain 42.4 grams of powder per liter of medium.
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
1 of 1
This Item | |||
|---|---|---|---|
| description suitable for plant cell culture, with sucrose and agar | description powder, suitable for plant cell culture | description powder, suitable for plant cell culture, with Gamborg′s vitamins | description 10 ×, liquid, suitable for plant cell culture |
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in - | shipped in ambient | shipped in ambient |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
7782-63-0 + 6484-52-2 + 10043-35-3 + 7791-13-1 + 10043-52-4
CAS No.
CAS No.
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Does the 1000mL label indicate that it can prepare 1000mL of reconstituted MS medium or is the 1000mL just the total volume of the powder? In essence, how much reconstituted MS medium could this actually prepare?
1 answer-
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寒天が入っているとのことですが、固形とはいえず、半分液状の緩い培地になってしまいました。どの程度、寒天が入っているのでしょうか。
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This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium. The product must be reconstituted in water and heated in order to dissolve the agar. Upon cooling, the medium will set up as a semi-solid gel.
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What are the steps to follow in preparing the culture medium for M9274 Basal medium?
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Preparing this product in a concentrated form is not recommended as some salt complexes may precipitate. Supplements may be added prior to sterilization or added aseptically to a sterile medium. Certain supplements (i.e. heat labile) may require filter sterilization and may affect the shelf life of the medium. The basic steps for preparing culture medium are the following: 1. Using a container twice the size of the desired final volume, measure out approximately 90% of the final required volume of tissue culture grade water (e.g. Sigma Product No. W-3500). Example: 900 ml for a final volume of 1000 ml. 2. While stirring, add the powdered product. 3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in Step 2. 4. Add desired supplements (e.g. auxins, cytokinins). 5. While stirring, adjust to the desired pH (e.g. 5.7 +/- 0.1) using KOH, NaOH, or HCl. 6. Add additional tissue culture grade water to bring the medium to the final volume. 7. Heat the solution to approx. 100o C while stirring. 8. Dispense the medium into culture vessels before or after autoclaving according to your application. Add heat labile constituents after autoclaving. 9. Sterilize the medium in a validated autoclave at 1Kg/cm2 (15 psi). The medium should attain a temperature of 121o C for at least 15 min. Refer to the Sigma Plant Cell Culture Catalog for recommended autoclave times for different volumes.
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Would an incorrect pH level affect the setting of the agar?
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Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
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M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
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This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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