M6435
Methionine Aminopeptidase from Pyrococcus furiosus
≥93% (SDS-PAGE), recombinant, expressed in E. coli
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| Size/SKU | Availability | Price |
|---|---|---|
0.02 unit | Please contact Customer Service for Availability | CZK 16,200.00 |
About This Item
Specific activity:
0.5 units/mg protein
Assay:
≥93% (SDS-PAGE)
Recombinant:
expressed in E. coli
CZK 16,200.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli
Quality Segment
assay
≥93% (SDS-PAGE)
form
solution
specific activity
0.5 units/mg protein
mol wt
37 kDa by SDS-PAGE
UniProt accession no.
foreign activity
Other proteases, none detected
shipped in
dry ice
storage temp.
−20°C
Gene Information
Pyrococcus furiosus DSM 3638 ... PF0541(1468383)
General description
X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein. [1]
Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.
Application
Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. [2] It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors.
Biochem/physiol Actions
Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.
Physical form
Solution containing 0.01% Tween® 20, 0.1 mM CoCl2, and 10 mM Tris-HCl, pH 7.5.
Other Notes
One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 °C.
Legal Information
TWEEN is a registered trademark of Croda International PLC
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 0.5 units/mg protein | specific activity ≥5.0 units/mg protein | specific activity ≥200 units/mg protein | specific activity ≥10,000 BAEE units/mg protein |
| assay ≥93% (SDS-PAGE) | assay ~90% (SDS-PAGE) | assay - | assay - |
| Gene Information Pyrococcus furiosus DSM 3638 ... PF0541(1468383) | Gene Information - | Gene Information - | Gene Information - |
| form solution | form lyophilized powder | form lyophilized powder | form essentially salt-free, lyophilized powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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