M6320

Monoclonal Anti-MAGE-1 antibody produced in mouse

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Melanoma-associatiated antigen 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03096998
• Conjugate: unconjugated
• Clone: MA454, monoclonal
• Application: ELISA (i), IHC, WB
• Citations: 9

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MA454, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~46 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 5-10 μg/mL using total cell extract of SKML37 cells
• isotype: IgG1
• UniProt accession no.: P43355
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAGEA1(4100)

Description

General description

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen.[1][2] MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28.[3] Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

Immunogen

recombinant human MAGE-1.

Application

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:

• ELISA
• immunoblotting
• immunohistochemistry

Biochem/physiol Actions

The antibody does not recognize MAGE-2 and MAGE-3.[4][5]

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC).[6] Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)