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M4892

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

5A basal medium

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Size/SKUAvailabilityPrice
10 L
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1 780,00 CZK

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

1 780,00 CZK


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Formulated to contain 11.9 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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M2154M2520M3769
description

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

description

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

form

liquid

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

500

Quality Level

500


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Storage Class

11 - Combustible Solids


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10043-52-4

CAS No.


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