M3692

Anti-MRP2 antibody, Mouse monoclonal

clone CPR96, purified from hybridoma cell culture

• Synonym(s): Anti-ABCC2, Anti-Multidrug Resistance Associated Protein 2, Anti-cMOAT, Anti-cMRP
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01865475
• Conjugate: unconjugated
• Clone: CPR96, monoclonal
• Application: ARR, ELISA (i), WB
• Citations: 8

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CPR96, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~180 kDa
• species reactivity: human
• concentration: ~1.5 mg/mL
• technique(s): indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using cell extracts of 293T cells transfected with human MRP2
• isotype: IgG1
• UniProt accession no.: Q92887
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ABCC2(1244)

Description

General description

Anti-MRP2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CPR96 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptideintestine.

The gene MRP2 (Multidrug Resistance-associated Protein 2), also referred to as ABCC2 (ATP binding cassette subfamily C member 2), encodes a glycosylated protein belonging to the subfamily of MRP efflux pumps, the members of which are localized exclusively to the apical membrane domain of polarized cells, such as hepatocytes, renal proximal tubule epithelia, and intestinal epithelia. The protein consists of 1545 aa residues having a molar mass of ∼190 kDa. The gene with 32 exons is mapped to human chromosome 10q24.

Application

Anti-MRP2 antibody, Mouse monoclonal has been used in:

• western blotting
• immunohistochemistry
• immunofluorescence microscopy
• enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Multidrug Resistance-associated Protein 2 (MRP2) contributes to bile flow and plays a detoxification role and provides protection against oxidative stress. Upregulation of MRP2 expression may be found in hepatocellular carcinomas.

The gene MRP2 (Multidrug Resistance-associated Protein 2) encodes a protein involved in organic anion efflux. It is involved in biliary transport and the efflux of many conjugated compounds across the apical membrane of the hepatocyte into the bile canaliculi. It also participates in multi-drug resistance in mammalian cells. Mutations in this gene have been associated with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by an increased concentration of bilirubin glucuronosides in blood.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids