M1395

IgG2b, Kappa from murine myeloma

clone MOPC 195, ascites fluid, lyophilized powder

• Synonym(s): Mouse IgG2b-κ
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00164802
• Conjugate: unconjugated
• Clone: MOPC 195, monoclonal
• Application: —
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• clone: MOPC 195, monoclonal
• form: lyophilized powder
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgG antibodies are mainly responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis .

Mouse IgG2b, Kappa is produced in the MOPC 195 tumor line , is a mineral oil-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.

The MOPC 195 tumor line that produces mouse IgG2b, k is a mineral oil-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.
The hapten binding specificity of the MOPC 195 line has not been determined. The ascites produced from the MOPC 195 tumor line contains IgG2b, k in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration.

Immunogen

Purified guinea pig IgG

Application

Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody (diluted 1: 30,000 in PBST with 0.2% BSA and 2% PVP) can be used in ELISA. It is also useful in western blotting and immunohistology.

Biochem/physiol Actions

Goat anti-guinea pig IgG (whole molecule) -alkaline phosphatase antibody reacts specifically with IgG of guinea pig.

Identity and purity of the antibody is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD). Electrophoresis of the ascites fluid followed by diffusion versus anti-mouse IgG2b and anti mouse kappa chain results in single arcs of precipitation. By ODD the ascites fluid (containing mouse IgG2b, k) is compared to purified myeloma protein.

Physical form

lyophilized powder

Preparation Note

To one vial of lyophilized powder, add 1 ml of deionized water and an appropriate antimicrobial agent (e.g. sodium azide) to a final concentration of 15 mM. Rotate vial gently until powder dissolves. Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use

Analysis Note

Each vial contains at least 5mg of mouse IgG2b, k myeloma protein, determined by densitometry of electrophoresed ascites fluid.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)