LUC1

Luciferase Reporter Gene Detection Kit

• NACRES: NA.84
• UNSPSC Code: 12352207
• Usage: kit sufficient for 100 assays
• Storage temp.: −70°C

Properties

• usage: kit sufficient for 100 assays
• Quality Segment: 200
• storage temp.: −70°C

Description

General description

Firefly luciferase is one of the most utilized reporter genes for the study of gene expression.[1] It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods.[2] This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg²⁺. The fluorescent product is quantified to measure its activity.[2]

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3
• hcodes: H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1