L9776

Leucine Aminopeptidase, microsomal from porcine kidney

suitable for protein sequencing, BioReagent, lyophilized powder

• Synonym(s): Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
• CAS Number: 9054-63-1
• UNSPSC Code: 12352200
• NACRES: NA.26
• EC Number: 232-942-5
• MDL number: MFCD00131501
• EC Number: 3.4.11.2 (BRENDA, IUBMB)

Properties

• product line: BioReagent
• form: lyophilized powder
• packaging: vial of 50 μg
• suitability: suitable for protein sequencing
• application(s): metabolomics; vitamins, nutraceuticals, and natural products
• storage temp.: −20°C

Description

General description

Leucine Aminopeptidase (LAP) is a proteolytic enzyme. It is an amino acid hydrolase that catalyzes the hydrolysis of leucine residues from the amino-terminal of proteins or peptides. LAP is naturally present in the liver, kidney, lung, and serum.

Application

Leucine Aminopeptidase has been used:

• in proteomics analysis
• as a treatment for protein depolymerization reaction
• to test its effect on the degradation of human recombinant IL-8

Biochem/physiol Actions

Leucine Aminopeptidase serves as a biomarker for drug-induced liver injury because in case of liver injury, cirrhosis, hepatitis, and other disorders serum LAP activity increases. Abnormal expression of LAP results in tumor cell proliferation, angiogenesis, and invasion, so it can also serve as a cancer-related biomarker.

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)