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L9017

Lectin from Phaseolus vulgaris (red kidney bean)

BioXtra, Phytohemagglutinin PHA-P, purified by affinity chromatography, lyophilized powder

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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Size/SKUAvailabilityPrice
1 mg
Please contact Customer Service for Availability
CZK 1,870.00
5 mg
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CZK 5,760.00
10 mg
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CZK 10,500.00

About This Item

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Biological source:
Phaseolus vulgaris

CZK 1,870.00

Please contact Customer Service for Availability
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biological source

Phaseolus vulgaris

product line

BioXtra

form

lyophilized powder

potency

≤17 μg per mL agglutination activity

purified by

affinity chromatography

impurities

salt, essentially free

storage temp.

2-8°C

General description

Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for phytohemagglutinin (PHA) swelling assay
  • for lymphocyte activation assay
  • to evaluate cell-mediated immune response

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.
Mitogenic activity at ≤10 μg per mL.

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This Item
L2769L8754L4144
Lectin from Phaseolus vulgaris (red kidney bean) BioXtra, Phytohemagglutinin PHA-P, purified by affinity chromatography, lyophilized powder

Sigma-Aldrich

L9017

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder

Sigma-Aldrich

L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L4144

Lectin from Phaseolus vulgaris (red kidney bean)

biological source

Phaseolus vulgaris

biological source

Phaseolus vulgaris

biological source

Phaseolus vulgaris

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

purified by

affinity chromatography

purified by

-

purified by

-

purified by

-

impurities

salt, essentially free

impurities

salt, free

impurities

salt, free

impurities

salt, free

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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