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L6522

Sigma-Aldrich

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

clone HP-6054, ascites fluid

Synonym(s):

Monoclonal Anti-Human Lambda Light Chains (Bound and Free)

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About This Item

MDL number:
MFCD00162516
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6054, monoclonal

contains

15 mM sodium azide

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Human Lambda Light Chains (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Specificity

The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse has been used:
  • in immunohistochemistry
  • in enzyme-linked immunosorbent assay (ELISA) assay
  • in immunohistology
  • in radioimmunoassay (RIA)
  • in precipitation assays
  • in immunoblotting
  • as capture antibodies in quantitative ELISA

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Primary lymphoma of the central nervous system: a clinical-pathological and immunohistochemical study of ten autopsy cases
Costa H, et al.
Arquivos de Neuro-Psiquiatria, 64(4), 976-982 (2006)
A human immunoglobulin lambda locus is similarly well expressed in mice and humans
Popov AV, et al.
The Journal of Experimental Medicine, 189(10), 1611-1620 (1999)
Patrizia Circelli et al.
Bioengineered bugs, 1(3), 221-224 (2011-02-18)
We have recently described an efficient transient expression system mediated by Agrobacterium tumefaciens for the production of HIV-1 Nef protein in Nicotiana benthamiana plants. In order to enhance the yield of recombinant protein we assayed the effect of three gene-silencing
Maria Elena Villani et al.
Plant biotechnology journal, 7(1), 59-72 (2008-09-17)
The aims of this work were to obtain a human antibody against the tumour-associated antigen tenascin-C (TNC) and to compare the yield and quality of plant-produced antibody in either stable transgenics or using a transient expression system. To this end
Jerry A Katzmann et al.
Clinical chemistry, 48(9), 1437-1444 (2002-08-27)
The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more

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