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Leucine Aminopeptidase, microsomal from porcine kidney

Name: Leucine Aminopeptidase, microsomal from porcine kidney
Brand: SIGMA
Price: 13200 CZK

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Synonym(s):

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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Size/SKU	Availability	Price
25 units	Please contact Customer Service for Availability	CZK 13,200.00
50 units	Please contact Customer Service for Availability	CZK 21,800.00
100 units	Please contact Customer Service for Availability	CZK 39,500.00

About This Item

CAS Number:

9054-63-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-942-5

MDL number:

MFCD00131501

EC Number:

3.4.11.2 (BRENDA, IUBMB)

Specific activity:

≥12 units/mg protein (biuret)

Biological source:

Porcine kidney

CZK 13,200.00

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Properties

biological source

Porcine kidney

type

Type VI-S

form

lyophilized powder

specific activity

≥12 units/mg protein (biuret)

mol wt

320 kDa

composition

Protein, 30-70%

storage temp.

−20°C

Description

General description

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.[1]

Biochem/physiol Actions

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Physical form

Contains primarily phosphate buffer salts, pH 7.0

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

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1 of 1

This Item	L5006	P6675	164598
Sigma-Aldrich L6007 Leucine Aminopeptidase, microsomal from porcine kidney	Sigma-Aldrich L5006 Leucine Aminopeptidase, microsomal from porcine kidney	Sigma-Aldrich P6675 Prolidase from porcine kidney	Sigma-Aldrich 164598 Aminopeptidase M, Porcine Kidney
specific activity ≥12 units/mg protein (biuret)	specific activity 10-40 units/mg protein (Bradford)	specific activity ≥100 units/mg protein	specific activity -
biological source Porcine kidney	biological source -	biological source -	biological source Porcine kidney
form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder	form suspension (crystalline)
mol wt 320 kDa	mol wt -	mol wt -	mol wt -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–2 of 2 Questions

Anonymous

a year ago

I would like to know the optimum solvent for dissolving, storing and using this reagent (Cat # L6007-25UN).

1 answer
1. Technical Support
  
  a year ago
  
  According to the Enzymatic Assay for Leucine Aminopeptidase, Microsomal Enzyme Solution, prepare a solution containing 0.10 - 0.15 unit/ml of Leucine Aminopeptidase, Microsomal in cold deionized water immediately before use.
  More information can be found at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/356/218/l6007enz.pdf.
  This formulation contains phosphate buffer salts. For reconstitution, storage, and use of this product, 50 mM Sodium Phosphate Buffer, pH7.2, is a suitable solvent.
  
  Helpful?
3 years ago

I recently purchased Leucine Aminopeptidase from porcine kidney Cat # L6007-25UN. I'd like to inquire how many mgs of protein is in one vial.

1 answer
1. Technical Support
  
  3 years ago
  
  The milligrams of protein per vial will depend on the units/mg protein defined on the product's certificate of analysis. For example, lot SLCM3898 reports specific activity of 19 units/mg protein. This results in approximately 1.3 mg of protein per vial for this particular lot. The protein content of this lot of material is reported at 61%, therefore the total mass of solid material in the 25UN vial will total around 2.2 mg.
  (Link to certificate of analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L6007-BULKSLCM3898.pdf)
  
  Helpful?

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