L5505

Lectin from Ulex europaeus (gorse, furze)

lyophilized powder

• Synonym(s): Ulex europaeus agglutinin, UEA-I
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Properties

• form: lyophilized powder
• Quality Segment: 200
• potency: <8 μg/mL agglutination activity
• composition: Protein, ≥80% biuret
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Preparation Note

Purified by affinity chromatography using the method of Pereira.[1]

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)