L3794

LOK (1-348), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): PRO2729, STK10
• NACRES: NA.32
• UNSPSC Code: 12352202
• Specific activity: 2-4 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 100
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 2-4 nmol/min·mg
• mol wt: ~65 kDa
• UniProt accession no.: O94804
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... STK10(6793)

Description

General description

LOK (lymphocyte-oriented kinase) belongs to the Ste20 (sterile 20) family of serine/threonine kinases. It is expressed in lymphocytes, brain, colon, thymus, kidney, liver, small intestine and lung.[1] LOK is commonly referred to as STK10 (serine/threonine protein kinase 10).

Biochem/physiol Actions

Mutations in LOK (lymphocyte-oriented kinase) are linked with the anti-apoptotic effects leading to carcinogenesis.[1] It functions as an ERM (ezrin-radixin-moesin) kinase and thereby regulates cytoskeletal organization and lymphocyte migration.[2] It can phosphorylate PLK1 (polo-like kinase 1).[3]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)