KCQS01

KiCqStart^® SYBR^™ Green qPCR ReadyMix^™

Low ROX^™, for ABI and Stratagene instruments

• Synonym(s): qPCR master mix, sybr green qPCR
• NACRES: NA.55
• UNSPSC Code: 41106300

Properties

• form: liquid
• usage: sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
• feature: dNTPs included, hotstart
• storage condition: protect from light
• technique(s): RT-qPCR: suitable, qPCR: suitable
• color: colorless
• input: purified DNA; crude DNA
• compatibility: for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio^™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
• detection method: SYBR^™ Green
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

KiCqStart^® SYBR^™ Green qPCR ReadyMix^™ has been used:

• for the amplification and quantification of DNA in real-time PCR (qPCR) assay[1]
• in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)[2]
• in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay[3]

PCR applications:

• Gene expression
• DNA quantification
• CHiP

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Analysis Note

Kit components are free of contaminating DNase and RNase. KiCqStart^® SYBR^™ Green qPCR ReadyMix^™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r² > 0.995) and a PCR efficiency > 90%.

Other Notes

2X reaction buffer containing optimized concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

QuantStudio is a trademark of IROA Technologies LLC

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable