I8773

Anti-Insulin-Like Growth Factor-I antibody produced in goat

affinity isolated antibody

• Synonym(s): Anti-IGF-I
• UNSPSC Code: 51111800
• NACRES: NA.41
• MDL number: MFCD00162566
• Conjugate: unconjugated
• Clone: polyclonal
• Application: Neutral, WB
• Citations: 8

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): neutralization: 3-12 μg/mL, western blot: 0.1-0.2 μg/mL
• UniProt accession no.: P05019
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... IGF1(3479)

Description

General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1 signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-IGF-I recognizes human insulin-like growth factor I. The antibody does not react with human IGF-II.

Immunogen

recombinant human IGF-I expressed in Escherichia coli

Application

Anti- IGF-1 antibody may be used for neutralization reactions, the ND50 of the antibody is 3-12 μg/ml. For immunoblotting a working antibody concentration of 0.1-0.2 μg/ml.

Biochem/physiol Actions

The antibody neutralizes the biological activity of recombinant human IGF-I.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose.

Preparation Note

Affinity isolated by IGF-I affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)