I8648

Interleukin-15 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-15
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00675504

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.2-4.0 ng/mL EC₅₀
• mol wt: antigen predicted mol wt ~12.9 kDa
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P40933
• storage temp.: −20°C
• Gene Information: human ... IL15(3600)

Description

Biochem/physiol Actions

IL-15 (interleukin 15) is a proinflammatory pleiotropic cytokine and is a connection between innate and adaptive immune responses. It participates in the proliferation, survival and activation of various lymphocytes lineages. IL-15 works by associating with a high affinity heterotrimeric receptor complex containing IL-15Rα, IL-2/15Rβ and IL-Rγ.[1][2][3] It is present in different forms, the soluble form, the hyper IL-15 formed by association between IL-15 receptor α chain and soluble cytokine, and the membrane bound isoform.[1]

Interleukin-15 was first isolated from the supernatant of a cultured simian kidney epithelial cell line.[4] The cDNA encodes a 162 amino acid precursor protein with a 48 amino acid signal peptide that is cleaved to yield a 114 amino acid mature protein. Human IL-15 shares sequence identity with simian (~97%) and murine (~73%). IL-15 competes for binding sites with IL-2, as both IL-2 and IL-15 stimulate the growth of cells through the IL-2 receptor.

Physical form

Lyophilized from a 0.2 μm filtered solution containing10 mM Sodium Bicarbonate, pH 8.5

Analysis Note

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)