I7153

Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01633547
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 165 kDa
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): 4 μg using 0.5 mg of a 3T3/A31 RIPA lysate, western blot: 0.5-2 μg/mL using RIPA lysates of 3T3/A31 mouse fibroblasts
• UniProt accession no.: P35568
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... IRS1(3667) ; mouse ... Irs1(16367) ; rat ... Irs1(25467)

Description

General description

Insulin receptor substrate 1 (IRS1) gene is mapped to human chromosome 2q36.3. The gene encodes a 185 kDa protein and is ubiquitously expressed. Irs1 belongs to the group of cytoplasmic adaptor proteins.

Immunogen

C-terminal 14 amino acid residues of rat liver insulin receptor substrate 1.

Application

Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in immunoprecipitation using 0.5 mg of a 3T3/A31 RIPA lysate.

Biochem/physiol Actions

Insulin receptor substrate 1 (IRS-1) is phosphorylated on multiple tyrosine residues. It plays a crucial role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways. Over expression of IRS-1can leads to mammary tumorigenesis and metastasis. Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in western blotting using RIPA lysates of 3T3/A31 mouse fibroblasts. Rabbit anti-Insulin Receptor Substrate 1 (IRS-1) antibody reacts specifically with insulin receptor substrate 1 (165 kD). The product has shown species cross reactivity with rat, mouse and human IRS-1.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl, and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable