H6161

[Gly¹⁴]-Humanin G human

≥95% (HPLC), powder

• Synonym(s): S14G-humanin, HNG
• UNSPSC Code: 12352209
• NACRES: NA.32
• MDL number: MFCD03787971

Properties

• biological source: human
• Quality Segment: 200
• assay: ≥95% (HPLC)
• form: powder
• mol wt: 2654.2-2660.2 Da
• storage condition: (Keep container tightly closed in a dry and well-ventilated place)
• technique(s): activity assay: suitable
• solubility: water: 1.00-1.04 mg/mL, clear, colorless
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: S(CC[C@H](N)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](Cc3ccccc3)C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCC(=O)N[C@@H](
• InChI: 1S/C118H202N34O31S2/c1-19-65(14)92(112(179)144-81(54-90(159)160)104(171)145-82(52-63(10)11)114(181)152-46-29-37-86(152)109(176)148-91(64(12)13)111(178)139-72(32-23-24-41-119)97(164)137-74(35-27-44-129-118(125)126)98(165)136-73(34-26-43-128-117(123)124)96(
• InChI key: WUYRHUFIUFJVQU-XJUNAYMOSA-N

Description

General description

Research area: Neuroscience

[Gly14]-humanin (HNG) belongs to the family of mitochondrial-derived peptides.[1] HNG (humanin-G) is an analog of humanin, generated by S14G (serine-14-glycine) mutation. HNG shows a 1000 times stronger activity.[2][3]

Application

[Gly14]-Humanin G human has been used:

• along with bortezomib in pharmacological inhibition studies to treat mice to limit the toxic effects of bortezomib[4][5]
• to study its effect on high glucose (HG)-induced apoptosis of endothelial cells (ECs)[6]
• to study its neuroprotective effects in an in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells[7]

[Gly¹⁴]-Humanin G human has been used to limit the toxicity during the bortezomib treatment in neonatal brachial plexus injury (NBPI) mice model. It has also been used in the cell culture of the human-derived neuroblastoma cell line.

Biochem/physiol Actions

Endogenous rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer′s disease genes and Aβ

Endogenous rescue factor abolishing neuronal cell death by many familial Alzheimer′s disease genes.

[Gly14]-humanin (HNG) stimulates cell growth, proliferation, and cell cycle progression. It enhances proteins such as kinases, transporters, transmembrane receptors, and transcription and translational regulators. HNG exhibits intense in vitro neuroprotective effects compared to natural HN.[8] Studies show that it is an effective cytoprotectant both in vitro and in vivo.[1][7] HNG is found to prevent high glucose (HG)-induced apoptosis of endothelial cells (ECs).[6] It is proven to inhibit AngII-induced phenotypic switch in vascular smooth muscle cells (VSMCs).[7]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)