It is recommended that fresh solutions be prepared before use. Stability testing of frozen solutions of this product has not been performed in our laboratories. One published reference cites storage of 1.0 mg/ml stock solutions of hyaluronidase in PBS at –20°C. Please see specific instructions on this chemical that will help in preparation. https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase
H3884
Hyaluronidase from bovine testes
Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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| Size/SKU | Availability | Price |
|---|---|---|
50 mg | Please contact Customer Service for Availability | CZK 2,290.00 |
100 mg | Please contact Customer Service for Availability | CZK 6,780.00 |
500 mg | Please contact Customer Service for Availability | CZK 13,500.00 |
1 g | Please contact Customer Service for Availability | CZK 23,100.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
750-3000 units/mg solid
Biological source:
bovine testis
biological source
bovine testis
Quality Segment
conjugate
conjugate (Glucosaminoglycan)
type
Type IV-S
form
lyophilized powder (essentially salt-free)
specific activity
750-3000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
application(s)
diagnostic assay manufacturing
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
cow ... HYAL1(515397), HYAL2(281838)
Biochem/physiol Actions
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Does 1mg/ml represent the minimum solution to have a 1mg of hyaluronidase powder completely dissolved? Can the entire containing-powder vial tolerate repeated defrost-thaw cycles?
1 answer-
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What should we set the specific activity of this product to? The range is 750-3,000 U/mg. We need 100 U/mL, how should we calculate it?
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The activity of this material is lot specific and reported in the Certificate of Analysis. The product may be reconstituted to the required concentration, based on the lot specific activity, using 20 mM sodium phosphate buffer, pH 7.0, with 77 mM NaCl and 0.1 mg/mL BSA. At the minimum activity, a 100 U/mL solution would be reconstituted with 7.5 mL of the recommended buffer, while at the maximum activity the volume would increase to 30 mL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/h3884#product-documentationPlease see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdfHelpful?
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How pure is the protein?
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Unfortunately, the purity of this product is not determined by test specifications.
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Can stock solutions of Product H3884, Hyaluronidase from bovine testes, be stored frozen after reconstitution?
1 answer-
Although Sigma-Aldrich has not tested the stability of stock solutions of Product H3884, Hyaluronidase from bovine testes, several publications cite storage of solutions of H3884 at -20 °C:1. Campbell, Keith H.S, "Transgenic Sheep form Cultured Cells", from Transgenesis Techniques: Principles and Protocols, Methods Mol. Biol., 180, 289-301 (2002). A 1500 IU/mL stock solution was stored in 20 mL aliquots at -20 °C.2. Marschall, S. and de Angelis, M.H., "In Vitro Fertilization/Cryopreservation", from Transgenic Mouse: Methods and Protocols, Methods in Molecular Biology, 209, pp. 35-50. Humana Press (2003). 1 mg/mL stock solutions in M2 medium or water, sterile-filtered (0.22 mm filter) can be stored in aliquots at -20 °C.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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