GEE70092Z

Shrimp Alkaline Phosphatase

Cytiva E70092Z, pack of 1000 U

• Synonym(s): AP, PCR buffer component, catalyst, dephosphorlyation enzyme, endonuclease buffer ingredient, phosphate hydrolase, protein
• NACRES: NA.54
• UNSPSC Code: 12352204

Properties

• form: solid
• packaging: pack of 1000 U
• manufacturer/tradename: Cytiva E70092Z
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Shrimp alkaline phosphatase is a high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8.0 to 8.5 by heating for 15 min at 65°C. No further treatment is necessary. The ALP (alkaline phosphatase) gene codes for a membrane-bound ectoenzymes that is present in four isoforms and is distributed as tissue nonspecific, intestinal, placenta, and germ cell ALP. The tissue nonspecific enzyme is ubiquitously expressed in a variety of tissues including liver, bone, kidney and is predominant in osteoblasts. Alkaline phosphatase is also known as mesenchymal stem cell antigen 1 or tissue nonspecific alkaline phosphatase.

Biochem/physiol Actions

Alkaline phosphatase hydrolyses pyrophosphate (PPi) to inorganic phosphate and thus maintains a balance between the two forms of phosphate which is highly essential for mineralization. Higher levels of serum alkaline phosphatase is found in prostate cancer. Severe TNAP deficiency refers to hypophosphatasia characterized with defective pyridoxal 5′-phosphate metabolism and skeletal hypomineralization.

Features and Benefits

• Removing 5′-phosphates from DNA and RNA.
• Easily inactivated by heat.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Safety Information

• Storage Class: 12 - Non Combustible Liquids