GE17-0409-03
HiTrap® Chelating High Performance
Cytiva 17-0409-03, pack of 5 × 5 mL
Synonym(s):
HiTrap Chelating Column
About This Item
Recommended Products
ligand
iminodiacetic acid
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0409-03
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
size
avg. part. size
34 μm
cleaning
2-14
working range
3-13
Related Categories
General description
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ Systems.
Features and Benefits
- For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service