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G8644

D-(+)-Glucose solution

100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

D-Glucopyranose

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Size/SKUAvailabilityPrice
100 mL
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CZK 1,790.00

About This Item

Empirical Formula (Hill Notation):
C6H12O6
CAS Number:
50-99-7
Molecular Weight:
180.16
MDL number:
MFCD00063774
UNSPSC Code:
12352201
PubChem Substance ID:
24895346
NACRES:
NA.75
Beilstein/REAXYS Number:
1281608

CZK 1,790.00

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sterility

sterile-filtered

Quality Segment

400

product line

BioXtra

form

solution

concentration

100 g/L in H2O

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

room temp

SMILES string

OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6+/m1/s1

InChI key

WQZGKKKJIJFFOK-DVKNGEFBSA-N

Gene Information

human ... PYGM(5837)

General description

Glucose provides the primary energy source for cell metabolism. It is a main source of energy for living organisms. Glucose occurs naturally in the free state in fruits and other parts of plants. It is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and starch), and glycogen.

Application

D-(+)-Glucose solution has been used:

  • to determine extracellular acidification rate (ECAR) or oxygen consumption rate (OCR) in human CB CD34+cells by Seahorse extracellular flux assay[1]
  • as a negative control in human umbilical vein endothelial cell (HUVEC) culture[2]
  • as a supplement for culturing colonic biopsies[3]

Biochem/physiol Actions

Glucose is an aldosic monosaccharide and acts as an important component of glycolysis and downstream pathways for aerobic and anaerobic respiration. It provides energy for growth and reproduction. Glucose is a byproduct of photosynthesis that is condensed to starch in plants and cyanobacteria.[4]

Other Notes

This product has been tested with cell lines to verify the product is not cytotoxic. The growth promoting capacity of this cell culture tested glucose solution was assessed at 45 mL/L in medium using appropriate cell lines.
This solution has been sterilized by filtration.

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This Item
G8769G7021G7528
D-(+)-Glucose solution 100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Sigma-Aldrich

G8644

D-(+)-Glucose solution

D-(+)-Glucose solution 45% in H2O, sterile-filtered, BioXtra, suitable for cell culture

Sigma-Aldrich

G8769

D-(+)-Glucose solution

D-(+)-Glucose powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

Sigma-Aldrich

G7021

D-(+)-Glucose

D-(+)-Glucose ≥99.5% (GC), BioXtra

Sigma-Aldrich

G7528

D-(+)-Glucose

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

gas chromatography (GC): suitable

Quality Level

400

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

solution

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

Insoluble matter, passes filter test

product line

BioXtra

product line

BioXtra

product line

BioReagent

product line

BioXtra

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Questions

1–5 of 5 Questions  

  1. Hello what is the recommended concentrations of D-glucose solution 100g/l to used for primary cell culture of brain ?

    1 answer

    1. There are a number of cell types present in brain tissue. Glucose requirements may vary by cell type. This product is not tested for use in primary cell culture. However, the typical glucose concentration in most classical media is 1 g/L or 5 g/L. In brain slice or hippocampal slice studies, the glucose concentration may vary broadly. It is advisable to search to the literature for the appropriate concentration of D-glucose to use for primary brain cell culture. Please see the links below to review examples of these publications:

      'Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology'
      https://www.sciencedirect.com/science/article/pii/S0278584612002862?via%3Dihub

      'Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease'
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964634/

      Helpful?

  2. What is the Molar concentration of Product G8769, D-(+)-Glucose solution?

    1 answer

    1. The molar concentration of Glucose solutions G8644 and G8769, may only be estimated. The glucose molecular weight is 180.16 g/mol, however the concentration is lot specific.

      Product G8644 has a concentration specification ranging from 90 - 110 g/L or 0.50 - 0.61 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8644#product-documentation

      Product G8769 has a concentration specification ranging from 405 - 495 g/L or 2.24 - 2.74 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8769#product-documentation

      Helpful?

  3. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the Molar concentration of Product G8644, D-(+)-Glucose solution?

    1 answer

    1. Based on a 10% (w/v) concentration and a molecular weight for glucose of 180 Da,  Product G8644 D-(+)-Glucose solution, is 0.56 M glucose.

      Helpful?

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