G6920
Endo-β-galactosidase from Bacteroides fragilis
recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution
Synonym(s):
β-Galactosidase bacterial, Keratanase
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| Size/SKU | Availability | Price |
|---|---|---|
0.5 unit | Please contact Customer Service for Availability | CZK 16,100.00 |
About This Item
Specific activity:
≥140 units/mg protein
Recombinant:
expressed in E. coli
CZK 16,100.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli
Quality Segment
conjugate
(Glucosaminoglycan)
sterility
aseptically filled
form
buffered aqueous solution
specific activity
≥140 units/mg protein
mol wt
32 kDa
storage temp.
2-8°C
Application
Biochem/physiol Actions
Internal β(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc β(1-3)Gal β (1-4)] structures are the preferred substrate.
Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfate
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.[3]
Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfate
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.[3]
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5
Other Notes
One unit will release 1.0 μmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥140 units/mg protein | specific activity ≥500 units/mg protein | specific activity ≥500 units/mg protein (biuret) | specific activity ≥20 units/mg protein |
| form buffered aqueous solution | form lyophilized powder | form aqueous glycerol suspension | form buffered aqueous solution |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant expressed in E. coli |
| sterility aseptically filled | sterility - | sterility - | sterility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 32 kDa | mol wt 465 kDa | mol wt 465 kDa | mol wt 80 kDa |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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