The molecular weight of this enzyme has not been determined. However, Biochem. Biophys. Res. Comm. 59, 894 (1974) reports a molecular weight of 55,000 daltons for the monomer. The enzyme is functionally active as a dimer or tetramer.
G5885
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, >= 550 units/mg protein (biuret)
Synonym(s):
G-6-P-DH
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| Size/SKU | Availability | Price |
|---|---|---|
200 units | Please contact Customer Service for Availability | CZK 1,860.00 |
1000 units | Please contact Customer Service for Availability | CZK 6,820.00 |
2000 units | Please contact Customer Service for Availability | CZK 9,840.00 |
10000 units | Please contact Customer Service for Availability | CZK 34,500.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
>= 550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
biological source
bacterial (Leuconostoc mesenteroides)
Quality Segment
type
Type XXIV
form
lyophilized powder
specific activity
>= 550 units/mg protein (biuret)
mol wt
128 kDa
composition
Protein, 15-40% biuret
application(s)
agriculture
foreign activity
6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%
storage temp.
2-8°C
General description
Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer.[1] Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.[2]
Application
Biochem/physiol Actions
Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.[2]
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Lyophilized powder containing Ficoll and Tris buffer salts
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity >= 550 units/mg protein (biuret) | specific activity ≥550 units/mg protein (biuret) | specific activity 550-1,100 units/mg protein (biuret) | specific activity 200-400 units/mg protein (modified Warburg-Christian) |
| biological source bacterial (Leuconostoc mesenteroides) | biological source - | biological source bacterial (Leuconostoc mesenteroides) | biological source - |
| application(s) agriculture | application(s) agriculture | application(s) - | application(s) diagnostic assay manufacturing |
| form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 128 kDa | mol wt - | mol wt 54 kDa | mol wt 128 kDa |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?
1 answer-
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What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
1 answer-
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli. Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
1 answer-
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).
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Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
1 answer-
Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.
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