G4877

Gonadotropin from pregnant mare serum

≥1,000 IU/mg

• Synonym(s): Equine gonadotropin, PMSG, Pregnant mare serum gonadotropin, eCG
• CAS Number: 9002-70-4
• UNSPSC Code: 12352202
• EC Number: 232-663-9
• MDL number: MFCD01779765

Properties

• form: powder
• specific activity: ≥1,000 IU/mg
• mol wt: 49- 68.5 kDa
• solubility: water: 1 mg/mL, clear to slightly hazy, colorless to faintly yellow
• storage temp.: −20°C

Description

General description

Gonadotropin hormone is only found in primates and is a member of the glycoprotein hormone family, which also consists of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). This hormone is composed of two non-covalently linked α and β subunits. The α-subunits within a species are identical, whereas the β subunits are different, which confers the differences in physiological specificity.

Application

Gonadotropin from pregnant mare serum has been used-

• for inducing superovulation in female Sprague Dawley rats
• for injection in female golden hamster to eventually obtain zona-free hamster eggs

Biochem/physiol Actions

Gonadotropin interacts with members of the GPCR (G-protein coupled receptor family), and upon activation they induce adenylyl cyclase.

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Stimulates the growth of ovarian follicles and the formation of the corpus luteum.

Two chain glycoprotein hormone; stimulates the growth of ovarian follicles and the formation of the corpus luteum.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges