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G4256

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

Synonym(s):

GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase

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Size/SKUAvailabilityPrice
20 units
Please contact Customer Service for Availability
CZK 22,900.00
50 units
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CZK 45,400.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
20-60 units/mg protein (modified Warburg-Christian)

CZK 22,900.00


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type

Type IV

Quality Segment

form

lyophilized powder

specific activity

20-60 units/mg protein (modified Warburg-Christian)

composition

Protein, 15-35%

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%, UDP glucose pyrophosphorylase and galactokinase ≤0.2%

storage temp.

−20°C

General description

Research area: Cell Signaling

Galactose-1-phosphate uridyltransferase (GALT) is a galactose metabolizing enzyme that facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity.[1] GALT gene is mapped to human chromosome 9p13. Deficiency of GALT results in type 1 galactosemia.[1]

Application

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast has been used to perform enzyme assays.

Physical form

Contains buffer salts as citrate and reduced glutathione

Other Notes

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

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This Item
SRE0008G5507U8501
specific activity

20-60 units/mg protein (modified Warburg-Christian)

specific activity

20-60 units/mg protein (modified Warburg-Christian)

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

specific activity

≥40 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

400

Quality Level

200

Quality Level

200

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%, UDP glucose pyrophosphorylase and galactokinase ≤0.2%

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%, UDP glucose pyrophosphorylase and galactokinase ≤0.2%

foreign activity

-

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%, inorganic pyrophosphatase ≤0.5%

composition

Protein, 15-35%

composition

Protein, 15-35%

composition

Protein, 35-65% modified Warburg-Christian

composition

Protein, 30-60% modified Warburg-Christian


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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