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G0411

Prionex® Highly purified Type A

aqueous solution

Synonym(s):

Gelatin, Prionex® Highly purified Type A

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Size/SKUAvailabilityPrice
100 mL
Please contact Customer Service for Availability
CZK 14,600.00
1 L
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CZK 88,200.00

About This Item

CAS Number:
9000-70-8
UNSPSC Code:
12352202
EC Number:
232-554-6
NACRES:
NA.61
MDL number:
MFCD00081638
Form:
aqueous solution
Biological source:
Porcine bone

CZK 14,600.00

Please contact Customer Service for Availability
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biological source

Porcine bone

Quality Segment

200

sterility

aseptically processed

form

aqueous solution

technique(s)

ELISA: suitable, microbiological culture: suitable, western blot: suitable

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue. Gelatin is a biocompatible polymer.

Application

Prionex® Highly purified Type A has been used:
  • for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA
  • as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry[1]
  • as a component of media for species differentiation in bacteriology[2]
  • as a delivery vehicle for the release of bioactive[3] molecules and in the generation of scaffolds for engineering applications[4]
  • in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo[5]

Legal Information

Prionex is a registered trademark of Pentapharm AG, Basel

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This Item
G139373865G9391
Prionex® Highly purified Type A aqueous solution

Sigma-Aldrich

G0411

Prionex® Highly purified Type A

Gelatin solution Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture

Sigma-Aldrich

G1393

Gelatin solution

Gelatin from Porcine skin, pkg of 500 g, suitable for microbiology

Millipore

73865

Gelatin

Gelatin from bovine skin Type B, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

G9391

Gelatin from bovine skin

technique(s)

ELISA: suitable, western blot: suitable, microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

biological source

Porcine bone

biological source

bovine hide

biological source

Porcine skin

biological source

bovine skin

form

aqueous solution

form

solution

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

sterility

aseptically processed

sterility

sterile

sterility

-

sterility

sterile

Storage Class

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–2 of 2 Questions  

  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is the shelf life for G0411 (purified prionex) after opening? And is it okay to store 2-8C?

    1 answer

    1. After opening, this product may be stored at 2-8°C. The solution may require warming to room temperature for ease of use. With proper storage and aseptic handling, the stability will not be impacted after opening. Any change in color or turbidity may be an indication of contamination or degradation.

      This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link:
      https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

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