F9175

Flt-3/Flk-2 Ligand from mouse

≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD01093789

Properties

• biological source: mouse
• Quality Segment: 100
• recombinant: expressed in NSO cells
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.0-4.0 ng/mL
• mol wt: ~18 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P49772
• storage temp.: −20°C
• Gene Information: mouse ... Flt3l(14256)

Description

General description

Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor.[1] Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.[2]

Mouse and human Flt-3 ligand share approximately 72% homology and both demonstrate cross-species activity.

Biochem/physiol Actions

Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myeloblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP and suppresses Erk signaling, which in turn promotes myogenesis.[1] In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.[2]

Physical form

Lyophilized from a 0.2 μm filtered solution in 50% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

Bioactivity is measured by the ability to stimulate the proliferation of a Flt-3 transfected pro-B cell line.

Heterogenous glycosylation causes recombinant mouse Flt-3 ligand to migrate as multiple bands from 18-32 kDa.

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)
• wgk: WGK 2