This product has not be tested for in vivo or in vitro use, but has been used successfully in mouse models by other researchers. Please review the PEER REVIEWED PAPERS above for list of publications. Please see the link below to review an example:
https://pmc.ncbi.nlm.nih.gov/articles/PMC5974208/
F7252
Fast Green FCF
Dye content ≥85 %, powder
Synonym(s):
Food green 3
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CZK 1,510.00
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CZK 6,190.00
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CZK 17,200.00
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About This Item
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
Molecular Weight:
808.85
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
EC Number:
219-091-5
MDL number:
Beilstein/REAXYS Number:
5718212
Colour Index Number:
42053
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Product Name
Fast Green FCF, Dye content ≥85 %
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
SMILES string
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
form
powder
composition
Dye content, ≥85%
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL, clear
Quality Level
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| solubility H2O: 1 mg/mL, clear | solubility H2O: 1 mg/mL | solubility - | solubility H2O: 1 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form powder | form powder | form - | form powder |
| composition Dye content, ≥85% | composition dye content, ≥90% | composition - | composition Dye content, ≥65% |
| mp 290 °C (dec.) (lit.) | mp 290 °C (dec.) (lit.) | mp 290 °C (dec.) (lit.) | mp 288 °C (dec.) (lit.) |
General description
Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.
Biochem/physiol Actions
Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
352.4 °F - closed cup
flash_point_c
178 °C - closed cup
ppe
Eyeshields, Gloves
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Synthetic Food Colors
Corradini MG
Encyclopedia of Agriculture and Food Systems, 291-296 (2019)
Fast green FCF alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model
Xu F, et al.
Frontiers in Pharmacology, 9, 534-534 (2018)
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Chevrier A, et al.
Osteoarthritis and Cartilage, 15(3), 316-327 (2007)
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Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
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Is Fast Green FCF (F7252) in vivo grade? Our lab wants to use it for retroorbital injections but wasn't sure if it was safe to be injected in live animals.
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Dear Sirs please advise why there is no expiration date in your product F7252 Lot.No. SHBN0187
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his product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information, please access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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