F4165
Ficin from fig tree latex
powder, ≥0.1 unit/mg solid
Synonym(s):
Debricin, Ficain, higueroxyl delabarre
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| Size/SKU | Availability | Price |
|---|---|---|
1000 units | Please contact Customer Service for Availability | CZK 2,840.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
Specific activity:
≥0.1 unit/mg solid
Biological source:
fig tree (latex)
CZK 2,840.00
Please contact Customer Service for Availability
biological source
fig tree (latex)
Quality Segment
form
powder
specific activity
≥0.1 unit/mg solid
mol wt
23.8 kDa
storage temp.
−20°C
General description
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Other Notes
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥0.1 unit/mg solid | specific activity ≥1.0 units/mg protein | specific activity ≥1.0 units/mg protein (biuret) | specific activity 1.5-10 units/mg solid |
| biological source fig tree (latex) | biological source fig tree (latex) | biological source fig tree (latex) | biological source - |
| form powder | form lyophilized powder | form saline suspension | form crude powder |
| mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt 23.4 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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