E8655

Anti-E6AP antibody, Mouse monoclonal

clone E6AP-330, purified from hybridoma cell culture

• Synonym(s): Anti-E2F-6
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD04034520
• Conjugate: unconjugated
• Clone: E6AP-330, monoclonal
• Application: ARR, ELISA (i), ICC, IP, WB
• Citations: 44

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: E6AP-330, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human, mouse, rat, monkey
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract from 293T cells
• isotype: IgG1
• UniProt accession no.: O75461
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... E2F6(1876) ; mouse ... E2f6(50496) ; rat ... E2f6(313978)

Description

General description

E6AP is an E3 ubiquitin ligase that is expressed by the UB3A gene. Inhibiton or alterations of the UB3A gene may cause a neurological disorder called the Angelman Syndrome. E6AP interacts with E1 and E2 enzymes to mediate ubiquitination of proteins marked for degradation. E6AP also binds with the E6 viral protein present in HPV-infected cells.
Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey.

Immunogen

human full-length recombinant E6AP.

Application

Monoclonal Anti-E6AP antibody is suitable for use in western blot (1-2 μg/mL using total cell extract from 293T cells), immunocytochemistry, immunoblotting, immunoprecipitation, indirect ELISA and microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable