E7407

ERK1, active, untagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MAPK3, P44ERK1, P44MAPK, PRKM3
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD03457429
• Specific activity: 329-445 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 329-445 nmol/min·mg
• mol wt: ~44 kDa
• UniProt accession no.: P27361
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAPK3(5595)

Description

General description

Extracellular signal-regulated kinase 1 (ERK1) belongs to mitogen-activated protein kinases (MAPK)/ERK family of intermediate proteins in the signaling pathways. This gene contains nine exons and eight introns, and spans 9kb. It is a constitutively expressed protein, the activity of which is altered by the phosphorylation state. It has a higher expression level in nervous tissues. It contains 11 kinase subdomains, common between humans and mice.[1] It has a molecular weight of 44kDa.[2]

Biochem/physiol Actions

ERK1 (MAPK3) participates in cellular signaling cascades that are activated in response to numerous growth factors and cytokines. Disruption of MAP kinase signaling functionality is associated with disease states including inflammation, cancer and neurodegenerative disease.

Extracellular signal-regulated kinase 1 (ERK1) act as intermediate in multiple signaling cascades, and is acted upon by MEK protein. MEK are essential for Golgi fragmentation during mitosis, and this is achieved by its action upon an alternatively spliced isoform of ERK1, called ERK1c.[3] ERK1/2 signaling plays a key role in cellular response to topoisomerase II poisoning, by activating G2/M checkpoint, and thus, prevents poison-induced cell death.[4] In melanocytes, pleiotrophin suppresses pigmentation or melanogenesis, by degradation of MITF, through the activation of ERK1/2 signaling.[5]

Physical form

Supplied in 25 mM MOPS, pH 7.5, with 300 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)