E2761

ExtrAvidin^®−FITC

Name: ExtrAvidin<SUP>®</SUP>−FITC
Brand: SIGMA
Price: 5070 CZK

buffered aqueous solution

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1 ML

CZK 5,070.00

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00163600

Form:

buffered aqueous solution

Key Documents

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conjugate

FITC conjugate

Quality Level

200

form

buffered aqueous solution

extent of labeling

3-6 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

shipped in

dry ice

storage temp.

−20°C

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This Item	E2636	A2050	E4011
Sigma-Aldrich E2761 ExtrAvidin^®−FITC	Sigma-Aldrich E2636 ExtrAvidin^® –Alkaline Phosphatase	Sigma-Aldrich A2050 Avidin–FITC from egg white	Sigma-Aldrich E4011 ExtrAvidin^®−R-Phycoerythrin
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200	technique(s) dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200	technique(s) flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
shipped in dry ice	shipped in ambient	shipped in dry ice	shipped in -
conjugate FITC conjugate	conjugate alkaline phosphatase conjugate	conjugate FITC conjugate	conjugate phycoerythrin (R-PE) conjugate
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[1] It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[2] ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Application

ExtrAvidin^®-FITC is suitable for the following applications:

immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.[3][4]
Immunofluorescence[5][6]†
competitive binding assay[7]

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number

0000519674

0000485835

0000485834

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Physiological and morphological properties of Dbx1-derived respiratory neurons in the pre-Botzinger complex of neonatal mice.

Maria Cristina D Picardo et al.

The Journal of physiology, 591(10), 2687-2703 (2013-03-06)

Breathing in mammals depends on an inspiratory-related rhythm that is generated by glutamatergic neurons in the pre-Bötzinger complex (preBötC) of the lower brainstem. A substantial subset of putative rhythm-generating preBötC neurons derive from a single genetic line that expresses the

Probing the ligand-binding domain of the mGluR4 subtype of metabotropic glutamate receptor.

D R Hampson et al.

The Journal of biological chemistry, 274(47), 33488-33495 (1999-11-24)

Metabotropic glutamate receptors (mGluRs) are G-protein-coupled glutamate receptors that subserve a number of diverse functions in the central nervous system. The large extracellular amino-terminal domains (ATDs) of mGluRs are homologous to the periplasmic binding proteins in bacteria. In this study

Expression and localization of urokinase-type plasminogen activator receptor in bovine cumulus--oocyte complexes

Garcia DC

Zygote, 24, 230-235 (2016)

In vivo PET imaging of the neuroinflammatory response in rat spinal cord injury using the TSPO tracer [(18)F]GE-180 and effect of docosahexaenoic acid.

J L Tremoleda et al.

European journal of nuclear medicine and molecular imaging, 43(9), 1710-1722 (2016-05-08)

Traumatic spinal cord injury (SCI) is a devastating condition which affects millions of people worldwide causing major disability and substantial socioeconomic burden. There are currently no effective treatments. Modulating the neuroinflammatory (NI) response after SCI has evolved as a major

Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice.

Xueying Wang et al.

eLife, 3, e03427-e03427 (2014-07-17)

To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm

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Global Trade Item Number

SKU	GTIN
E2761-1ML	04061838163219
E2761-.2ML	04061838124043

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ExtrAvidin^®−FITC