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E2412

Sigma-Aldrich

Pullulanase microbial

Synonym(s):

Pullulanase microbial, Promozyme® D2

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50 ML
CZK 3,700.00
250 ML
CZK 9,630.00

CZK 3,700.00

Please contact Customer Service for Availability
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50 ML
CZK 3,700.00
250 ML
CZK 9,630.00

About This Item

CAS Number:
9075-68-7
Enzyme Commission number:
3.2.1.41 (BRENDA, IUBMB)
MDL number:
MFCD00081941
UNSPSC Code:
12352204
NACRES:
NA.54

CZK 3,700.00

Please contact Customer Service for Availability

recombinant

expressed in Bacillus subtilis

Quality Level

200

form

aqueous solution

specific activity

≥1000 NPUN/g

storage temp.

2-8°C

Application

Pullulanase is a glucanase that degrades pullulan. It is commonly used for starch-debranching as well as to study polysaccharide utilization[1].

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

A product of Novozymes Corp.
Novozym is a registered trademark of Novozymes A/S
Promozyme is a trademark of Novozymes Corp.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000373134
0000305950
0000305951
0000258717
0000258717

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Nasima Chorfa et al.
Polymers, 14(10) (2022-05-29)
In this work, a valorization of the starch stemming from downgraded potatoes was approached through the preparation of starch nanoparticles using different physical methods, namely liquid and supercritical carbon dioxide, high energy ball milling (HEBM), and ultrasonication on the one
Christopher C Ibenegbu et al.
Microbial cell factories, 21(1), 251-251 (2022-11-30)
The starch in waste bread (WB) from industrial sandwich production was directly converted to ethanol by an amylolytic, ethanologenic thermophile (Parageobacillus thermoglucosidasius strain TM333) under 5 different simultaneous saccharification and fermentation (SSF) regimes. Crude α-amylase from TM333 was used alone
K A Smith et al.
Journal of bacteriology, 171(4), 2116-2123 (1989-04-01)
We have cloned a pullulanase gene from Bacteroides thetaiotaomicron. The pullulanase expressed from this clone in Escherichia coli was cell associated and soluble and had a molecular mass of 72 kilodaltons by gel filtration. Maxicell analysis of proteins coded by
Edward K Gilding et al.
Nature communications, 4, 1483-1483 (2013-02-14)
The production of adequate agricultural outputs to support the growing human population places great demands on agriculture, especially in light of ever-greater restrictions on input resources. Sorghum is a drought-adapted cereal capable of reliable production where other cereals fail, and
S J King
Molecular oral microbiology, 25(1), 15-24 (2010-03-25)
Streptococcus pneumoniae colonization of the human airway is an essential precursor for disease; however, the mechanisms by which the bacterium establishes and maintains colonization are poorly understood. It is becoming increasingly clear that S. pneumoniae expresses glycosidases that can modify
View All Related Papers

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Questions

1–3 of 3 Questions  

  1. May I know how to make 10units/mL sample from the bottle?

    1 answer

    1. The activity in the batch-specific Certificate of Analysis is expressed as NPUN/g. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN, where 1 PUN is defined as the amount of enzyme that releases 1 μmol of glucose per minute under standard conditions.

      The Certificate of Analysis also reports the density (g/mL) of the product. To calculate the activity in units per milliliter (UPUN/mL), multiply the activity (NPUN/g) by the density (g/mL). Further dilution can then be performed to achieve the desired final concentration.

      Helpful?

  2. I was wondering if product E2412-50ML is a type 1 or type 2 pullulanase (degrades alpha1,6 linkages only or both alpha1,4 and 1,6?)

    1 answer

    1. E2412 contains both Type 1 and Type 2 Pullulanases.

      Helpful?

  3. What is the molecular weight of this Pullulanase enzyme? And is there any glucose in the enzyme solution?

    1 answer

    1. The molecular weight has not been determined, however, a search of the literature indicates that the general molecular weight of Pullulanase, CAS 9075-68-7, ranges from 70 - 115 kDa. This is a product of Novozymes Corp. The manufacturer considers the formulation of this product proprietary but has noted that this material is pure Oligo-1,6-Glucosidase.

      Helpful?

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