Elastase from porcine pancreas is soluble in 200 mM Tris HCl buffer, pH 8.8 (1 mg/ml), yielding a clear solution. For storage, lyophilized elastase is stable at -20°C; however, the stability of the solution at -20°C has not been validated.
E0127
Elastase from porcine pancreas
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Please contact Customer Service for Availability | CZK 4,410.00 |
10 mg | Please contact Customer Service for Availability | CZK 7,900.00 |
20 mg | Please contact Customer Service for Availability | CZK 13,300.00 |
100 mg | Please contact Customer Service for Availability | CZK 53,700.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
254-453-6
MDL number:
Specific activity:
≥4.0 units/mg protein
type
Type III
Quality Segment
form
lyophilized powder
specific activity
≥4.0 units/mg protein
mol wt
25.9 kDa
composition
Protein, 55-85%
solubility
200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear
storage temp.
−20°C
Application
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.
Packaging
Package size based on protein content
Physical form
Contains sodium carbonate.
Preparation Note
2x crystallized and chromatographically purified
Analysis Note
Contains trypsin activity
Other Notes
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥4.0 units/mg protein | specific activity ≥25 units/mg protein | specific activity ≥4.0 units/mg protein (biuret) | specific activity ≥4.0 units/mg protein |
| form lyophilized powder | form lyophilized | form lyophilized powder | form suspension |
| solubility 200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear | solubility distilled water: 10 mg/mL | solubility - | solubility - |
| mol wt 25.9 kDa | mol wt - | mol wt - | mol wt 25.9 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the stability of solubled elastaze in 200 mM Tris HCl (1mg/ml) in -20 Celsius degrees?
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