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D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Synonym(s):
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
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5 SETS
CZK 1,300.00
50 SETS
CZK 5,640.00
CZK 1,300.00
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name
SIGMAFAST™ DAB with Metal Enhancer, tablet
form
tablet
solubility
water: 1 tablet/5 mL
storage temp.
−20°C
Quality Level
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| solubility water: 1 tablet/5 mL | solubility water: 1 tablet/5 mL | solubility water: 0.5 tablet/10 mL, clear, colorless | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form tablet | form tablet | form tablet |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
General description
SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Application
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
Preparation Note
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Saba Shahin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)
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M Breijo et al.
Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)
The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 175, 254-268 (2017-09-20)
Birds time their daily and seasonal activities in synchronization with circadian and annual periodicities in the environment, which is mainly provided by changes in photoperiod/day length conditions. Photoperiod appears to act at the level of eye, pineal and encephalic/deep brain
Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Keith Morris-Schaffer et al.
Particle and fibre toxicology, 16(1), 1-1 (2019-01-08)
Recent epidemiological studies indicate early-life exposure to pollution particulate is associated with adverse neurodevelopmental outcomes. The need is arising to evaluate the risks conferred by individual components and sources of air pollution to provide a framework for the regulation of
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocols
Tento protokol slouží k provedení celého postupu imunohistochemie (IHC) prostřednictvím barvení a vizualizace specifických antigenů v parafinových řezech tkání.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Related Content
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
1 answer-
This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
Helpful?
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What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 answer-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
Helpful?
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How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 answer-
Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
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