This product is described as intact human chorionic gonadotropin (hCG) consisting of α and β subunits. Hyperglycosylated hCG or other isoforms are not specifically tested for in this product, and the ratio of free β subunit to the whole molecule is not controlled or tested.
CG10
Chorionic gonadotropin human
lyophilized powder, vial of ~10,000 IU
Synonym(s):
Choriogonin, HCG
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | CZK 8,780.00 |
10 vials | Please contact Customer Service for Availability | CZK 36,100.00 |
About This Item
Biological source:
human urine
Form:
lyophilized powder
biological source
human urine
Quality Segment
sterility
sterile-filtered
form
lyophilized powder
mol wt
37.9 kDa
contains
mannitol
packaging
vial of ~10,000 IU
drug control
Home Office Schedule 4.2
impurities
HBsAg, HCV and HIV-1/HIV-2, tested negative
solubility
water: 10 mL/vial, clear to slightly hazy, colorless
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... CGA(1081)
General description
Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG.
Application
Chorionic gonadotropin has been used:
- in in vitro analysis of cytotoxicity
- in egg and embryo preparations
- in the superovulation of CD-1 mice
- in gonadotropin preparations for the in vivo studies with the luteinizing hormone-releasing hormone (LHRH) agonist (LHRH-a) to synchronize naturally cycling rats.
Biochem/physiol Actions
Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals.[1] When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Preparation Note
When reconstituted with 10 mL water to a concentration of 1,000 IU/mL, the vial will also contain 0.01 M sodium phosphate buffer (pH approx. 7.2) and 10 mg/mL of mannitol.
Analysis Note
The calculated value is based on the USP method for human Chorionic Gonadotrophin, to meet 10,000 IU per vial, with a tolerance of not less than 80.0% and not more than 125.0% of the potency stated.
1 of 1
This Item | |||
|---|---|---|---|
| biological source human urine | biological source human urine | biological source human urine | biological source human urine |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| solubility water: 10 mL/vial, clear to slightly hazy, colorless | solubility water: 10 mL/vial, clear, colorless | solubility water: 10 mL/vial, clear to slightly hazy, colorless | solubility H2O: 1 mL/vial, saline: 1 mL/vial |
| Gene Information human ... CGA(1081) | Gene Information human ... CGA(1081) | Gene Information human ... CGA(1081) | Gene Information human ... CGA(1081) |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in dry ice |
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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hCGにはhyperglycosylated hCGなど様々なアイソフォームが存在すると思いますが、本製品は全てを内包したものと考えて良いのでしょうか？もしくはintact hCGのみでしょうか？またそれを調べた検証結果などありますでしょうか？
1 answer-
Helpful?
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Hi, I would like to ask if it is possible to know the protein content of 1 vial in mg or µg. thank you
1 answer-
Each vial of CG10-1VL contains approximately 10,000 International Units (IU) of human chorionic gonadotropin (hCG). This product is lyophilized from solution and dispensed based on activity, rather than protein concentration. The protein content is not determined. Each vial contains 100 mg Mannitol and 0.01 M sodium phosphate buffer.
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Could you please confirm whether this product is Intact hCG? We are required to perform a cross-reactivity study for Free Beta hCG, and we would like to know if this product can be used for that purpose.
1 answer-
The product CG10 is intact human chorionic gonadotropin (hCG) composed of both α and β subunits. It is not free β hCG. The product is purified but may contain some contaminating proteins/hormones and is not specifically tested for cross-reactivity with free β hCG. Therefore, CG10 is not suitable as a free β hCG standard for cross-reactivity studies.
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wie lange kann man das CG10 lagern?
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This product is a lyophilized powder. The recommended long term storage temperature is -20°C. This item is stable for short periods and is shipped at ambient temperatures. There is no assigned expiration date for the lyophilized powder. While the material is quite stable, this item is warranted for a period of 1 year from the date of shipment. Upon reconsitution in water the material may be stored in aliquots at -20°C. Avoid repeated freeze/thaw cycles. Please see the link below to review the product datasheet for additional information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/245/988/cg10pis.pdfHelpful?
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