The RPMI 8866 cell line datasheet is accessible through the cell line catalogue entry on the website. Our ECACC cell culturists have recommended that this cell line might require up to 7 days to reach the exponential or log phase of the cell cycle. Once it reaches this phase, the doubling time of the cell line is expected to be around 18-24 hours, provided the cells are seeded at the optimal subculture seeding density of between 2-3 x 10^4 cells/ml.
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About This Item
UNSPSC Code:
41106514
Biological source:
human blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Lymphoblastoid
Manufacturer/tradename:
ECACC
Pricing and availability is not currently available.
biological source
human blood
manufacturer/tradename
ECACC
packaging
tube of 5 μg 95041316-DNA-5UG, pkg of vial of cells 95041316-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Immunoglobulin E (IgE) binding factor, Calcitonin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Receptor studies, binding assays, hormone studies.
Biochem/physiol Actions
Human B lymphocyte
RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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This Item | |||
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95041316, human blood, Lymphoblastoid | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 91062702, mouse blood, Macrophage | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91051511, mouse blood, Not specified |
| growth mode Suspension | growth mode Suspension | growth mode Semi-adherent | growth mode Semi-adherent |
| karyotype Not specified | karyotype Not specified | karyotype Not specified | karyotype Not specified |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| packaging tube of 5 μg 95041316-DNA-5UG, pkg of vial of cells 95041316-1VL | packaging pkg of vial of cells 87012702-1VL, tube of 5 μg 87012702-DNA-5UG | packaging tube of 5 μg 91062702-DNA-5UG, vial of cells 91062702-1VL | packaging pkg of vial of cells 91051511-1VL, tube of 5 μg 91051511-DNA-5UG |
| biological source human blood | biological source human blood | biological source mouse blood | biological source mouse blood |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
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Could you provide information on product 95041316-1VL, including the doubling time and data sheet details?
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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What medium should I use when I thaw the Cell Line cells?
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The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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