HT29 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91072201, human colon, Epithelial

• Synonym(s): HT29 Cells
• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46, hypertriploid
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 91072201-DNA-5UG, pkg of vial of cells 91072201-1VL
• growth mode: Adherent
• karyotype: 2n = 46, hypertriploid
• morphology: Epithelial
• products: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

Biochem/physiol Actions

Human Caucasian colon adenocarcinoma

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Preparation Note

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.