4-11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Synonym(s): HeLa
• UNSPSC Code: 41106514
• Biological source: human cervix
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human cervix
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 11033103-DNA-5UG, pkg of vial of cells 11033103-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Description

Biochem/physiol Actions

4-11 is a HeLa cell line stably transfected with two constructs: the human androgen receptor (hAR) expression construct; a firefly luciferase reporter gene under the control of the MMTV promoter. 4-11 can be used to detect androgenic activity of chemicals.

HeLa, genetic modification, human androgen receptor, firefly luciferase reporter gene, MMTV promoter

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Preparation Note

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL + 800 μg/ml G418.

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Legal Information

US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.