This is a cell line. Please see the following 3 citation links: https://www.cellosaurus.org/CVCL_2459 and https://pubmed.ncbi.nlm.nih.gov/?term=h1RPE7 and https://scholar.google.com/scholar?hl=en&as_sdt=0%2C26&q=h1rpe7&btnG=
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Product Name
h1RPE7, from human eye, 09061602
biological source
human eye
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Cell Line Origin
An immortalized retinal pigment epithelial cell line
Cell Line Description
The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
Culture Medium
Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 24hrs.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Certificates of Analysis (COA)
Lot/Batch Number
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Hi I'd like to inquire about the h1RPE7 cell line. There's little documentation and no reviews. Is it indeed the cell line? The product name "09061602-DNA-5UG" sounds very much like a DNA-sample... Kind regards, Elmar
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