KARPAS 422

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Synonym(s): KARPAS, 422
• UNSPSC Code: 41106514
• Biological source: human lymph (lymphoma)
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Hyperdiploid with 10% polyploidy
• Morphology: Round polygonal cells growing singly or in small clusters in suspension
• Manufacturer/tradename: ECACC

Properties

• biological source: human lymph (lymphoma)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 06101702-DNA-5UG, pkg of vial of cells 06101702-1VL
• growth mode: Suspension
• karyotype: Hyperdiploid with 10% polyploidy
• morphology: Round polygonal cells growing singly or in small clusters in suspension
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: -140 to -196°C

Description

Biochem/physiol Actions

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).

Human B cell non-Hodgkin lymphoma

Not specified

Preparation Note

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO₂; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.