CASP8C

Caspase 8 Assay Kit, Colorimetric

• NACRES: NA.84
• UNSPSC Code: 12352202

Properties

• shipped in: dry ice
• Quality Segment: 200
• storage temp.: −20°C
• Gene Information: human ... CASP8(841)

Description

General description

Caspases (cysteine-requiring aspartate proteases) belong to a highly conserved family of cysteine proteases with specificity for aspartic acid residues found in their substrates. Caspases play a central role in apoptosis. Inhibition of caspases prevents the cell death process. Currently, there are 13 caspases recognized in mammals. Caspases are classified as initiators and effectors. Caspase 8 belongs to the class of initiators. It possesses a prodomain at the terminal and contains the death effector domain which serves as protein-protein interaction motif for caspase 8. With respect to substrate specificity, caspase 8 is classified as a member of group III. It specifically recognizes the (I/L/V) EXD sequence on the substrate.

Application

The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:

• purified caspase 8 for a positive control
• caspase 8 inhibitor
• p-NA fluorescent standard

Preparation Note

The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.

Kit Components Only

• N-Acetyl-Ile-Glu-Thr-Asp-al, ≥97% (HPLC) 100 μL
• Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL
• 10X Assay Buffer 5 mL
• 5X Lysis Buffer 5 mL

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable