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C9134

α-Chymotrypsin−Agarose from bovine pancreas

lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

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50 units
Please contact Customer Service for Availability
8 210,00 CZK
6 978,50 CZK
100 units
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14 600,00 CZK

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.21.1 (BRENDA, IUBMB)
MDL number:
MFCD00130481
Specific activity:
2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)
Biological source:
bovine pancreas

6 978,50 CZK

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biological source

bovine pancreas

Quality Segment

form

lyophilized powder

specific activity

2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

mol wt

25 kDa

UniProt accession no.

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

Application

α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

Physical form

Stabilized with lactose

Analysis Note

Protein determined by A1%/280

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 8.0 at 30 °C.

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This Item
C7762C4129C6423
α-Chymotrypsin−Agarose from bovine pancreas lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

Sigma-Aldrich

C9134

α-Chymotrypsin−Agarose from bovine pancreas

α-Chymotrypsin from bovine pancreas Type I-S, essentially salt-free, lyophilized powder

Sigma-Aldrich

C7762

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas Type II, lyophilized powder, ≥40 units/mg protein

Sigma-Aldrich

C4129

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas suitable for protein sequencing, salt-free, lyophilized powder

Sigma-Aldrich

C6423

α-Chymotrypsin from bovine pancreas

description

lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

description

Type I-S, essentially salt-free, lyophilized powder

description

Type II, lyophilized powder, ≥40 units/mg protein

description

suitable for protein sequencing, salt-free, lyophilized powder

biological source

bovine pancreas

biological source

-

biological source

-

biological source

-

specific activity

2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

specific activity

≥40 units/mg protein

specific activity

≥40 units/mg protein

specific activity

-

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

salt-free, lyophilized powder

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

P00767

mol wt

25 kDa

mol wt

25 kDa

mol wt

25 kDa

mol wt

25 kDa

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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