C8743

Cholesterol Dehydrogenase from Nocardia sp.

• CAS Number: 67775-34-2
• MDL number: MFCD03788777
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.1.1.B40
• Specific activity: ≥18 unit/mg solid
• Recombinant: expressed in Streptomyces lividans

Properties

• recombinant: expressed in Streptomyces lividans
• Quality Segment: 200
• form: lyophilized solid
• specific activity: ≥18 unit/mg solid
• mol wt: ~37 kDa by SDS-PAGE
• storage temp.: −20°C

Description

Application

Enzyme used in determining cholesterol in serum

The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.

Biochem/physiol Actions

Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.

Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.

Physical form

Supplied as a lyophilized powder

Other Notes

One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)