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Merck

C8679

Chicago Sky Blue 6B

powder

Synonym(s):

Direct Blue 1, Pontamine sky blue

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About This Item

Empirical Formula (Hill Notation):
C34H24N6Na4O16S4
CAS Number:
Molecular Weight:
992.80
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
24410
NACRES:
NA.47
EC Number:
220-026-8
MDL number:

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InChI key

BPHHNXJPFPEJOF-GPTZEZBUSA-J

InChI

1S/C34H28N6O16S4.4Na/c1-55-23-11-15(3-7-19(23)37-39-21-9-5-17-25(57(43,44)45)13-27(59(49,50)51)31(35)29(17)33(21)41)16-4-8-20(24(12-16)56-2)38-40-22-10-6-18-26(58(46,47)48)14-28(60(52,53)54)32(36)30(18)34(22)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

SMILES string

[Na+].[Na+].[Na+].[Na+].COc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(OC)c4

form

powder

composition

Dye content, ~80%

color

blue

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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This Item
A5268A3157M5528
color

blue

color

blue

color

dark blue

color

-

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

H2O: 10 mg/mL

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL, blue

solubility

H2O: 1 mg/mL, blue

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

composition

Dye content, ~80%

composition

Dye content, 45-65%

composition

Dye content, ≥50%

composition

-

Application

Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry.[1] It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.[2][3]

Biochem/physiol Actions

Chicago Sky Blue 6B is a large organic acid, structurally related to glutamate, that is a potent and efficient competitive inhibitor of vesicular glutamate uptake.[4]

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

2610-05-1

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number

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Responses to tactile stimulation in deep cerebellar nucleus neurons result from recurrent activation in multiple pathways.
Rowland NC and Jaeger D
Journal of Neurophysiology, 99, 704-704 (2008)
Li Hang Qi et al.
Human gene therapy, 20(7), 739-750 (2009-03-13)
Because both Toll-like receptor-1 (TLR1) and TLR2 are expressed in atherosclerotic plaques, we hypothesized that TLR1 and TLR2 may play different roles in the formation of vulnerable plaques and that combinatorial knockdown of TLR1 and TLR2 genes may enhance the
Detection of aromatase in human endometrial tissue cultured in three-dimensional fibrin matrix in vitro.
Jafar A, et al.
International journal of reproductive biomedicine (Yazd, Iran), 7, 105-109 (2009)
T Cowen et al.
Histochemistry, 82(3), 205-208 (1985-01-01)
The stain pontamine sky blue (PSB) has been shown to reduce background autofluorescence in catecholamine fluorescence and immunofluorescence histochemical preparations. Using PSB as a counterstain on whole-mount stretch preparations of human mesenteric blood vessels, a medium dense noradrenergic nerve plexus
Resonant antidromic cortical circuit activation as a consequence of high-frequency subthalamic deep-brain stimulation.
Li S, et al.
Journal of Neurophysiology, 98, 3525-3525 (2007)

Questions

  1. Hi, I need to know information to Chicago Sky Blue - is available declaration of conformity for this stain? New requirtments regulation of IVDR.

    1 answer
    1. This product is intended for research use only. It is not suitable for In Vitro Diagnostic use. The only documents available for this item are the product Certificate of Analysis, Certificate of Origin, and Safety Data Sheet are routinely available.
      Please see the link below to review additional information regarding product Quality Level Assignments and the associated documentation for each level.
      https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/m-clarity-program

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