C7806

Bovine Collagen Type II

from bovine nasal septum, powder, suitable for ELISA assays

• Synonym(s): Collagen from bovine
• CAS Number: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.61
• EC Number: 232-697-4
• MDL number: MFCD00130825
• Form: powder
• Biological source: bovine nasal septum

Properties

• Product Name: Collagen from bovine nasal septum, Bornstein and Traub Type II, powder
• biological source: bovine nasal septum
• Quality Segment: 200
• form: powder
• technique(s): ELISA: suitable
• solubility: aqueous acid: soluble
• UniProt accession no.: P02459
• storage temp.: 2-8°C
• Gene Information: bovine ... COL2A1(407142)

Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is a fibrous protein, found in connective tissues, hard tissues, bone, dentin, cementum and also the mineralizing cartilage of the epiphyseal growth plate.

Application

Collagen-type II has been investigated for its importance in haemostasis. Two peptides, CB8 and CB11, are sites of platelet binding and activation on collagen-type 2. Collagen-type 2 has also been used as a model for arthritis in animals, where FR140423, a selective cyclooxygenase (COX)-2 inhibitor, has shown an anti-inflammatory effect without gastric lesions that may occur with treatment via indomethacin.

Collagen Type II is sometimes used to induce an animal model of rheumatoid arthritis. However, under conditions leading to formation of reactive oxygen species, the subjects develop tolerance for auto-antibodies. This is an unusual situation of ROS having a favorable effect.

Collagen from bovine nasal septum has been used to coat the plates to estimate the levels of serum anti-cII IgG by enzyme-linked immunosorbent assay (ELISA). It has also been used as a component in the emulsified agent to induce arthritis in rats.

Biochem/physiol Actions

Collagen is the structural constituent of the dermis, which provides strength and support to the human skin. Variations in collagen participates in the aging process.

Preparation Note

An acid soluble collagen soluble in dilute acetic acid at pH 3.0 (5 mg/ml), yielding an opalescent, colorless solution.

Collagen Type II is a triple helix composed of three α1(II) strands.

Prepared by a modification of the pepsin extraction procedure of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)