This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.
C7527
Choline chloride
BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
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Synonym(s):
(2-Hydroxyethyl)trimethylammonium chloride
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| Size/SKU | Availability | Price |
|---|---|---|
100 g | Please contact Customer Service for Availability | 1 850,00 CZK |
1 kg | Please contact Customer Service for Availability | 5 160,00 CZK |
500 g | Please contact Customer Service for Availability | 5 340,00 CZK |
About This Item
Linear Formula:
(CH3)3N(Cl)CH2CH2OH
CAS Number:
Molecular Weight:
139.62
UNSPSC Code:
12352205
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-655-4
Beilstein/REAXYS Number:
3563126
MDL number:
biological source
synthetic (organic)
Quality Segment
product line
BioReagent
assay
≥98%
form
crystals
mol wt
Mw 139.62 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white
pH
5.0-6.5 (25 °C, 140 g/L)
mp
302-305 °C (dec.) (lit.)
solubility
H2O: 50 mg/mL
SMILES string
[Cl-].C[N+](C)(C)CCO
InChI
1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1
InChI key
SGMZJAMFUVOLNK-UHFFFAOYSA-M
General description
Listen to an Audio Overview
Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.
Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.
Choline acetyltransferase substrate
Application
Choline chloride has been used:
- as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
- for choline standard curve generation for quantifying phospholipase D (PLD) activity
- as a component of slice solution for the dissection of entorhinal-hippocampal brain slices
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This Item | |||
|---|---|---|---|
| description BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98% | description Pharmaceutical Secondary Standard; Certified Reference Material | description FCC | description ≥98% |
| technique(s) cell culture | mammalian: suitable, cell culture | insect: suitable, cell culture | plant: suitable | technique(s) gas chromatography (GC): suitable, HPLC: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| assay ≥98% | assay - | assay - | assay ≥98% |
| biological source synthetic (organic) | biological source - | biological source synthetic | biological source synthetic |
| form crystals | form - | form powder, crystals or chunks | form powder or crystals |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility - |
| mp 302-305 °C (dec.) (lit.) | mp 302-305 °C (dec.) (lit.) | mp 302-305 °C (dec.) (lit.) | mp 302-305 °C (dec.) (lit.) |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?
1 answer-
Helpful?
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Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?
1 answer-
This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w
Helpful?
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Hi, could you please provide the product information sheet for Choline chloride.
1 answer-
There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:
Certificate of Analysis
https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdfCertificate of Origin
https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921VHelpful?
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