C4249

Cystatin B human

recombinant, expressed in E. coli, solid, ≥95% (SDS-PAGE)

• Synonym(s): CPI-B, CST6, CSTB, EPM1, Liver Thiol Proteinase Inhibitor, PME, STFB, Stefin B
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Cystatin B human, recombinant, expressed in E. coli
• recombinant: expressed in E. coli
• Quality Segment: 200
• description: contains a N-terminal 7X His-tag
• assay: ≥95% (SDS-PAGE)
• form: solid
• mol wt: apparent mol wt ~13 kDa by SDS-PAGE (reducing)
• NCBI accession no.: NP_000091
• storage temp.: −20°C
• Gene Information: human ... CSTB(1476)

Description

General description

Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).

Application

Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.

Biochem/physiol Actions

Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain. PMID 12393805

Preparation Note

Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable